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Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
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Cancer Research
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JoVE Journal Cancer Research
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

DOI:
10.3791/64199-v

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06:29 min

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April 07, 2023

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, , , , ,
1Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine

Chapters

  • 00:04Introduction
  • 01:11Separation of Nuclear and Cytoplasmic Fractions
  • 01:56Validation of Nuclear-Cytoplasmic Separation
  • 04:35Results: Confirmation of RNA Purity of Cytoplasmic and Nuclear Fraction
  • 05:48Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol offers an efficient and flexible method to isolate RNA from nuclear and cytoplasmic fractions using cultured cells, and then validate using qPCR. This effectively serves as a replacement for other RNA preparation kits.

Tags

Keywords: Cytoplasmic RNANuclear RNACell FractionationLysis BufferQPCRMALAT1TUG1Cell CultureIntracellular InteractionsNuclear-cytoplasmic ExchangeCancer Development

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