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体内
啮齿动物膨胀诱发尿路上皮ATP释放的管腔测量
JoVE Journal
Medicine
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JoVE Journal
Medicine
In Vivo
Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents
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体内
啮齿动物膨胀诱发尿路上皮ATP释放的管腔测量
DOI:
10.3791/64227-v
•
09:17 min
•
September 07, 2022
•
Stephanie L. Daugherty
,
Keara M. Healy
,
Jonathan M. Beckel
1
Department of Pharmacology and Chemical Biology
,
University of Pittsburgh School of Medicine
Chapters
00:05
Introduction
00:35
Anesthesia and Ganglionic Block
01:29
Surgical Procedure: Suprapubic Bladder Catheterization
02:29
Transurethral Catheterization
03:43
Experimental Setup
05:08
Collection of Samples
06:20
Quantifying ATP from Collected Samples
07:03
Results: Measurement of Urothelial ATP Release In Vivo from the Lumen of the Bladder
08:39
Conclusion
Summary
Automatic Translation
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该协议描述了在麻醉啮齿动物中测量膀胱腔中ATP浓度的程序。
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