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High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
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JoVE Journal Chemistry
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

DOI:
10.3791/64252-v

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07:48 min

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September 30, 2022

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, , , , ,
1Department of Chemistry,Clemson University, 2Department of Biological Sciences,Clemson University, 3Department of Bioengineering,Clemson University

Chapters

  • 00:04Introduction
  • 00:54Initialize the System and Acquire a Plain Radiograph
  • 02:25Performing a Background Scan with the X-Ray Off
  • 03:07Performing a Sample Scan with the X-Ray on and Forming the Image
  • 04:00Culturing Bacteria for Imaging in Sterile Conditions
  • 05:53Results: Imaging the Intramedullary Rod Sensor in a Reamed Tibia of a Rabbit
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for high-resolution optical detection of chemical information around implanted medical devices with X-ray excited luminescence chemical imaging (XELCI). This novel imaging technique is developed in our lab which enables studying implant-associated infection biochemistry.

Tags

Chemical ImagingX-ray Excited LuminescenceImplant-associated InfectionsBiochemical ChangesHigh Spatial ResolutionBacterial CulturingRadiographyPoly Capillary OpticsX-ray SourceX-ray CameraImaging SystemScan Parameters

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