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Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo
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JoVE Journal Immunology and Infection
Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

DOI:
10.3791/64299-v

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07:33 min

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September 28, 2022

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, , , , , , , , , , , ,
1National Engineering Research Centre of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy,Third Military Medical University of Chinese PLA

Chapters

  • 00:04Introduction
  • 00:49Preparation of the I‐OVA NE
  • 01:33In Vitro and In Vivo Toxicity Assays
  • 05:25Results: In Vitro and In Vivo Experimental Evaluation of the Nasal Tumor Nanovaccine Delivery
  • 07:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present detailed methods for the preparation and evaluation of the nasal self-assembled nanoemulsion tumor vaccine in vitro and in vivo.

Tags

Keywords: Nasal Self-assembled NanoemulsionTumor VaccineIn VitroIn VivoMonophosphorol Lipid ADMSOTWEEN 80I-OVASqualeneMPLABEAS2B Epithelial CellsPBSTrypsinRPMI 1640

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