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人细胞中HSV-1感染后RIPK3和MLKL的ZBP1依赖性磷酸化的免疫荧光荧光放大的酪胺信号扩增
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Immunology and Infection
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JoVE Journal Immunology and Infection
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
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人细胞中HSV-1感染后RIPK3和MLKL的ZBP1依赖性磷酸化的免疫荧光荧光放大的酪胺信号扩增

DOI:
10.3791/64332-v

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09:15 min

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October 20, 2022

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, , ,
1VIB-UGent Center for Inflammation Research, 2Department of Biomedical Molecular Biology,Ghent University

Chapters

  • 00:05Introduction
  • 00:36Seeding and Stimulation of the Cells
  • 01:55Fixing the Cells
  • 02:32Permeabilization and Primary Staining
  • 03:42Tyramide Signal Amplification (TSA)
  • 04:58Fluorophores
  • 05:44Results: Tyramide Signal Amplification During Immunofluorescent Staining
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

免疫荧光染色期间的酪胺信号扩增能够在 HSV-1 感染后 ZBP1 诱导的坏死性凋亡期间灵敏地检测磷酸化的 RIPK3 和 MLKL。

Tags

Keywords: Tyramide Signal AmplificationImmunofluorescent StainingZBP1PhosphorylationRIPK3MLKLNecroptosisHSV-1Human CellsMicroscopyCell CultureFixationPermeabilizationAntibody Staining

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