Journal
/
Biochemistry
/
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

DOI:
10.3791/64340-v

•

08:27 min

•

November 30, 2022

•

, ,
1School of Chemistry, Chemical Engineering, and Biotechnology,Nanyang Technological University, 2State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering,Zhejiang University of Technology, 3Institute for Digital Molecular Analytics and Science,Nanyang Technological University

Chapters

  • 00:04Introduction
  • 00:37Cleaning of Nanochip
  • 01:54Generation of Small Unilamellar Vesicles (SUVs)
  • 02:35Formation of the SLB on a Nanochip
  • 03:53Imaging the SLB and the Curvature Sensing Protein Binding on the Nanochip
  • 05:52Results: Protein Curvature Sensing Characterization and Membrane‐Binding Dynamics of Proteins Around the Nanobar
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a nanobar-supported lipid bilayer system is developed to provide a synthetic membrane with a defined curvature that enables the characterization of proteins with curvature sensing ability in vitro.

Tags

Nanobar-supported Lipid BilayerMembrane Curvature SensingProtein DynamicsNanofabricationPiranha SolutionLipid MixtureSUVsPDMS Chamber

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64340?article_type=v

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
check_url/64340?article_type=v

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
check_url/64340?article_type=v

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
check_url/64340?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/64340?article_type=v

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
check_url/64340?article_type=v

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
check_url/64340?article_type=v

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
check_url/64340?article_type=v

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
check_url/64340?article_type=v

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
check_url/64340?article_type=v

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Read Article