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利用利什曼原虫破译毛孔形成毒素的分子机制和功能
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
DOI:

08:17 min

October 28, 2022

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Chapters

  • 00:05Introduction
  • 01:12Cytotoxicity Assay
  • 06:24Results: Tyrode s Buffer Sensitivity to L. major Proteins Compared to M199 and MEK Pathway Activation in L. major
  • 07:40Conclusion

Summary

Automatic Translation

这里介绍的是使用 利什曼原虫主要 前鞭毛体来确定孔形成毒素诱导的结合、细胞毒性和信号传导的方案。提供了链球菌溶血素O的概念验证。其他毒素也可用于利用 杆菌 中可用的遗传突变体来确定毒素抗性的新机制。

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