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Flowcytometribasert kvantifisering og analyse av myokardceller
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
DOI:

12:46 min

August 17, 2022

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Chapters

  • 00:04Introduction
  • 01:05Intravascular B-Cell Staining and Heart Collection
  • 02:58Heart Digestion and Cell Staining
  • 05:35Flow Cytometry
  • 11:31Results: Representative Plots Reporting the Average Number of B-Cells Per Milligram of Myocardial Tissue
  • 12:12Conclusion

Summary

Automatic Translation

Her rapporterer vi en protokoll for kvantifisering og differensiering av myokardiale B-lymfocytter basert på deres plassering i det intravaskulære eller endotelialrommet ved hjelp av flowcytometri.

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