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The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
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Bioengineering
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JoVE Journal Bioengineering
The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

DOI:
10.3791/64377-v

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08:50 min

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March 10, 2023

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, , ,
1Department of Chemical Engineering,Ben Gurion University of the Negev, 2Department of Chemical Engineering, Ilse Kats Institute for Nanoscale Science and Technology,Ben Gurion University of the Negev

Chapters

  • 00:05Introduction
  • 00:59Cleaning, Hydrophilization, and Silanization
  • 02:30Surface Passivation with an Inert Polymer
  • 03:20Preparing Myosin II Motor Aggregates and Actomyosin Solution
  • 04:45Running an Experiment
  • 05:23Microscopy Techniques
  • 06:36Results: Fulfillment of the Poroelasticity Aims
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

In this work, an in vitro reconstitution approach is employed to study the poroelasticity of actomyosin gels under controlled conditions. The dynamics of the actomyosin gel and the embedded solvent are quantified, through which the network poroelasticity is demonstrated. We also discuss the experimental challenges, common pitfalls, and relevance to cell cytoskeleton mechanics.

Tags

Keywords: ActomyosinPoroelasticCytoskeletonRheologyIn VitroCoverslip PreparationSurface PassivationPiranha SolutionSalinizationMethoxypoly(ethylene Glycol) Maleimide

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