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Journal / Bioengineering / Fabrication of Extracellular Matrix-derived Foams …
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Bioengineering

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JoVE Journal Bioengineering
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
 

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Article DOI: 10.3791/55436-v • 11:19 min • April 11th, 2017
April 11th, 2017 •
1, 2, 3, 2,4
1Biomedical Engineering Graduate Program, The University of Western Ontario, 2Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, The University of Western Ontario, 3Department of Chemical Engineering, Queen's University, 4Department of Chemical & Biochemical Engineering, Faculty of Engineering, The University of Western Ontario

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Summary
Automatic Translation

The tissue-specific extracellular matrix (ECM) is a key mediator of cell function. This article describes methods for synthesizing pure ECM-derived foams and microcarriers that are stable in culture without the need for chemical crosslinking for applications in advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.

Tags

Keywords: Extracellular Matrix ECM Foams Microcarriers Tissue Engineering 3D Cell Culture Tissue-specific Decellularization Cryomilling Alpha Amylase NaH2PO4 Buffer NaCl Wash
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