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Chapters
- 00:04Introduction
- 00:51Expression of ncAA‐Bearing Proteins in Salmonella
- 02:22In Vitro Fluorescence Labeling of ncAA‐Bearing Proteins in Salmonella Typhimurium
- 03:27Bio‐Orthogonal Labeling of Salmonella Secreted Effector SseJ‐F10TCO‐HA in HeLa Cells
- 08:08Super‐Resolution (dSTORM) Imaging
- 09:57Results: Incorporation of TCO*A into SseJ‐F10TCO‐HA Expressed in Escherichia coli and Salmonella, and Super‐Resolution Imaging of GCE‐Labeled Secreted SseJ‐J10TCO‐HA in HeLa Cells
- 12:27Conclusion
This article provides a straightforward and clear protocol to label Salmonella secreted effectors using genetic code expansion (GCE) site-specifically and image the subcellular localization of secreted proteins in HeLa cells using direct stochastic optical reconstruction microscopy (dSTORM)
