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使用遗传密码扩增对细菌分泌蛋白进行超分辨率成像
JoVE Journal
Biology
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JoVE Journal Biology
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
DOI:

13:11 min

February 10, 2023

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Chapters

  • 00:04Introduction
  • 00:51Expression of ncAA‐Bearing Proteins in Salmonella
  • 02:22In Vitro Fluorescence Labeling of ncAA‐Bearing Proteins in Salmonella Typhimurium
  • 03:27Bio‐Orthogonal Labeling of Salmonella Secreted Effector SseJ‐F10TCO‐HA in HeLa Cells
  • 08:08Super‐Resolution (dSTORM) Imaging
  • 09:57Results: Incorporation of TCO*A into SseJ‐F10TCO‐HA Expressed in Escherichia coli and Salmonella, and Super‐Resolution Imaging of GCE‐Labeled Secreted SseJ‐J10TCO‐HA in HeLa Cells
  • 12:27Conclusion

Summary

Automatic Translation

本文提供了一种简单明了的方案,使用遗传密码扩展 (GCE) 位点特异性标记 沙门氏菌 分泌效应子,并使用直接随机光学重建显微镜 (dSTORM) 对 HeLa 细胞中分泌蛋白的亚细胞定位进行成像

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