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Journal
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Biochemistry
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一种内部构建的基于发光二极管的光动力治疗设备,用于增强维替泊芬在 2D 细胞培养模型中的细胞毒性
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
Please note that all translations are automatically generated.
Click here for the English version.
一种内部构建的基于发光二极管的光动力治疗设备,用于增强维替泊芬在 2D 细胞培养模型中的细胞毒性
DOI:
10.3791/64391-v
•
11:04 min
•
January 13, 2023
•
Isadora da Silva Zanzarini
,
Gustavo Barbosa
,
Larissa de Oliveira Prado
,
Ingrid Fatima Zattoni
,
Gustavo Da Paz
,
Ademir Luiz do Prado
3
,
Waldemar Volanski
,
Marcos Dinís Lavarda
,
Fabiane Gomes de Moraes Rego
,
Geraldo Picheth
,
Vivian Rotuno Moure
,
Glaucio Valdameri
1
Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance
,
Federal University of Parana
,
2
Graduate Program in Pharmaceutical Sciences
,
Federal University of Parana
,
3
Federal Institute of Parana
Chapters
00:02
Introduction
00:40
Device Construction
03:10
Cell Lines: Cultivation, Seeding, and Treatment
05:19
Device Operation
06:56
Cell Viability Assays
07:47
Representative Results
09:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种新颖,简单且低成本的设备,可以使用二维HeLa细胞培养物和维特泊芬作为光敏剂成功进行
体外
光动力疗法(PDT)测定。
Tags
Photodynamic Therapy
PDT
Light-emitting Diode
LED
Verteporfin
Cytotoxicity
2D Cell Culture
In-house-built Device
PhotoAct
Low-cost
HeLa Cells
Dulbecco’s Modified Eagle Medium
Cell Culture
Article
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