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Chapters
- 00:04Introduction
- 00:48Preparation of the O-Ring
- 01:37Premedication of the Mice and Preparation of the Operating Field
- 02:10Intubation of the Mice
- 03:33Surgery and Preparation for Ring Positioning
- 04:41Ligation of the Transverse Aorta with the O-Ring
- 05:41Suture and Post-Operative Recovery
- 06:33Confirmation of Successful Constriction and Right Position of the Ring
- 08:08Results: Modeling Pressure Overload-Induced Cardiac Hypertrophy Using the O-Ring Aortic Banding in a Murine Model
- 08:57Conclusion
The present protocol describes a new technique of aortic banding in mice to induce pressure overload cardiac hypertrophy. For banding, a rubber ring with a fixed inner diameter is used. This new technique promises less variance and more reproducible data for future experiments.
