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用于体外研究缺血性中风的人血脑屏障的三重原代细胞培养模型

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

Please note that all translations are automatically generated. Click here for the English version.

用于体外研究缺血性中风的人血脑屏障的三重原代细胞培养模型

DOI:

10.3791/64469-v

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11:06 min

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October 06, 2022

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Nikolai Fattakhov, Silvia Torices, Sarah Becker, Timea Teglas, Oandy Naranjo, Michal Toborek²

¹Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, ²Institute of Physiotherapy and Health Sciences,The Jerzy Kukuczka Academy of Physical Education

Chapters

00:05Introduction
00:50Triple Cell Culture BBB Model Setting
03:35Induction of the Oxygen-Glucose Deprivation
05:19TEER Measurements
06:49Assessment of the BBB Paracellular Permeability
07:40Detection of Hypoxia in Live Cells
08:41Results: A Method to Set up an In Vitro Reliable Triple Endothelial Cell-Pericyte-Astrocyte Culture BBB Model
10:20Conclusion

Summary

Automatic Translation

在这里，我们描述了基于原代人脑微血管内皮细胞，星形胶质细胞和周细胞建立血脑屏障三重细胞培养模型的方法。该多细胞模型适用于 体外缺 血性卒中期间神经血管单元功能障碍的研究或候选药物的筛选。

Tags

Keywords: Blood-brain Barrier Ischemic Stroke In Vitro Model Triple Cell Culture Pericytes Astrocytes Cell Culture Protocol Cell Density Trypsinization Cell Counting Cell Seeding

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