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Chapters
- 00:04Introduction
- 00:57Crude Plastoglobule Extraction from Un-Stressed Maize Leaf Tissue
- 04:41Crude Plastoglobule Extraction from Cyanobacteria
- 07:06Harvesting Pure Plastoglobules
- 07:55Cyanobacteria Processing
- 08:43Results: Isolation of Plastoglobule Lipid Droplets Associated with Various Photosynthetic Organisms
- 09:36Conclusion
A fast and efficient protocol is presented for the isolation of plastoglobule lipid droplets associated with various photosynthetic organisms. The successful preparation of isolated plastoglobules is a crucial first step that precedes detailed molecular investigations such as proteomic and lipidomic analyses.
