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Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System
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JoVE Journal Neuroscience
Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System

Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System

DOI:
10.3791/64530-v

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05:43 min

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October 21, 2022

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, , , , ,
1Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Research Institute of Integrated TCM & Western Medicine,Chengdu University of Traditional Chinese Medicine, 3School of Pharmacy,Chengdu University of Traditional Chinese Medicine, 4School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine

Chapters

  • 00:04Introduction
  • 00:44Brain Microdialysis Probe Implantation Surgery
  • 02:24Microdialysis System Connection and Probe Check
  • 03:15Collection of Hippocampal Extracellular Fluid (HECF) from the Awake Rat
  • 04:02Maintenance of the Microdialysis System and Devices After Sampling
  • 04:36Results: Analysis of HECF Collected Using the Microdialysis Sampling Equipment
  • 04:54Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol here provides a detailed real-time dynamic sampling of extracellular fluid from the hippocampus of awake rats using a microdialysis system.

Tags

Hippocampal Extracellular FluidBrain MicrodialysisReal-time Dynamic CollectionConscious RatsCentral Nervous DiseasesStereotaxic Brain LocatorCranial DrillMicrodialysis CasingACSFMulti-channel Swivel ControllerMicrosyringe Pump

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