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Journal
/
Medicine
/
离体
尿路上皮细胞中机械活化的CA
2+
瞬变分析
JoVE Journal
Medicine
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JoVE Journal
Medicine
Ex Vivo
Analysis of Mechanically Activated Ca
2+
Transients in Urothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
离体
尿路上皮细胞中机械活化的CA
2+
瞬变分析
DOI:
10.3791/64532-v
•
05:35 min
•
September 28, 2022
•
Marcelo D. Carattino
2
,
Wily G. Ruiz
,
Gerard Apodaca
2
1
Renal-Electrolyte Division, Department of Medicine
,
University of Pittsburgh
,
2
Department of Cell Biology
,
University of Pittsburgh
Chapters
00:04
Introduction
00:31
Fabrication of Micropipettes
00:56
In Situ Transduction and Isolation of Bladder Mucosa
01:41
Mechanical Stimulation of Individual Urothelial Cells and Ca
2+
Imaging
03:16
Data Analysis
04:03
Results: Analysis of Mechano-Activated Ca
2+
Transients in Urothelial Cells
04:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种使用荧光Ca
2 +
传感器GCaMP5G评估天然尿路上皮细胞中机械激活离子通道功能的方法。
Tags
Ex Vivo
Mechanically-activated
Ca2+ Transients
Urothelial Cells
Micropipettes
Bladder
Urethra
Calcium Ion Transients
Electrophysiology
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