Journal
/
Cancer Research
/
Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

DOI:
10.3791/64535-v

•

06:08 min

•

February 10, 2023

•

, , , , , , , ,
1T Cell Engineering Laboratory,Mayo Clinic, Rochester, 2Division of Hematology,Mayo Clinic, Rochester, 3Mayo Clinic Graduate School of Biomedical Sciences,Mayo Clinic, Rochester, 4Department of Molecular Medicine,Mayo Clinic, Rochester, 5Regenerative Sciences PhD Program,Mayo Clinic, Rochester, 6Department of Immunology,Mayo Clinic, Rochester

Chapters

  • 00:04Introduction
  • 00:49Assessment of CART19 Cell‐Associated Toxicities
  • 01:33MRI Imaging
  • 04:02Results: Assessment of CART Cell‐Associated Toxicities in a PDX Mice Model
  • 05:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol in which an acute lymphoblastic leukemia patient-derived xenograft model is used as a strategy to assess and monitor CD19-targeted chimeric antigen receptor T cell-associated toxicities.

Tags

Keywords: CAR-TAcute Lymphoblastic LeukemiaPatient-derived XenograftToxicity AssessmentMotor WeaknessWeight LossCytokine AnalysisMRI ImagingBreathing MonitoringParaVision Software

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64535?article_type=v

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
check_url/64535?article_type=v

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
check_url/64535?article_type=v

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
check_url/64535?article_type=v

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
check_url/64535?article_type=v

Assessment of the Metabolic Profile of Primary Leukemia Cells
check_url/64535?article_type=v

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
check_url/64535?article_type=v

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
check_url/64535?article_type=v

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
check_url/64535?article_type=v

A Melanoma Patient-Derived Xenograft Model
check_url/64535?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Read Article