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In Vivo Real-Time Study of Drug Effects on Carotid Blood Flow in the Ovine Fetus
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Developmental Biology
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JoVE Journal Developmental Biology
In Vivo Real-Time Study of Drug Effects on Carotid Blood Flow in the Ovine Fetus

In Vivo Real-Time Study of Drug Effects on Carotid Blood Flow in the Ovine Fetus

DOI:
10.3791/64551-v

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11:59 min

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April 28, 2023

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, ,
1School of Animal and Comparative Biomedical Sciences, College of Agriculture and Life Sciences,University of Arizona, Tucson

Chapters

  • 00:00Introduction
  • 00:48Surgical Procedure
  • 06:58Placing the Fetus Back and Closing the Wound
  • 08:21Post-Operative In Vivo Experiments
  • 09:28Results: Localized Effects of Blood Flow Manipulation in Near-Term and Preterm Fetus and Sheep
  • 11:06Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a method to deliver drugs and gene expression-modifying agents perivascularly in an in utero developing fetus. Importantly, the effect of drugs/agents on blood flow can be measured with the progression of pregnancy.

Tags

In VivoReal-timeCarotid Blood FlowOvine FetusWire MyographyOrgan BathsChronic ExposureLong-term EvaluationArterial ContractilityBlood FlowFetal PositionCarotid ArteryPerivascular CatheterSternocleidomastoid MusclePlatysmaOmohyoid MuscleFlow Probe

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