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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

DOI:
10.3791/64565-v

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10:27 min

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October 21, 2022

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, , ,
1Institute of Biological Sciences, Faculty of Science,Universiti Malaya, 2Centre for Research in Biotechnology for Agriculture (CEBAR),Universiti Malaya

Chapters

  • 00:04Introduction
  • 01:02CircRNA Predictions
  • 01:46Differential Expression Analysis and Filtering of DE circRNAs
  • 03:26Predicting the circRNA-miRNA Interaction Using Circr
  • 05:05Construction of the ceRNA Network
  • 06:34Functional Enrichment Analysis
  • 07:49Results: Functional Analysis of DE circRNAs Parental Genes Using GO and KEGG Enrichment
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol submitted here explains the complete in silico pipeline needed to predict and functionally characterize circRNAs from RNA-sequencing transcriptome data studying host-pathogen interactions.

Tags

Keywords: CircRNAHost-pathogen InteractionsIn Silico IdentificationCharacterizationBioinformaticsRNA-seq AnalysisDifferential Expression AnalysisCiriquantPrepDE.pyCiri DE ReplicateLinuxProgrammingProtocolWorkflow

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