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Immunology and Infection

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Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

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Immunology and Infection
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JoVE Journal Immunology and Infection

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

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Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

DOI:

10.3791/64566-v

•

07:12 min

•

June 23, 2023

•

Ricardo Gonçalves, Gabriela Kaliff Teófilo Murta, Izabela Aparecida de Souza, David M. Mosser

¹Department of General Pathology, Institute of Biological Sciences,Federal University of Minas Gerais, ²Department of Cell Biology and Molecular Genetics,University of Maryland

Chapters

00:00Introduction
01:08Removal of the Femur and Tibia
01:44Isolation of Bone Marrow Progenitors from Tibia and Femur
03:07Macrophage Culture
03:57Harvesting Macrophages
05:39Results: Characterizing the Bone Marrow-Derived Macrophages
06:42Conclusion

Summary

Automatic Translation

The present protocol describes the isolation and culture of bone marrow-derived macrophages from mice.

Tags

Bone Marrow Macrophages Isolation Culture Murine C57BL/6 Sterile Technique Centrifugation Cell Suspension DMEM/F12

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