/

/

使用延时显微镜和蛋白质的阶段特异性核耗竭来研究酿酒酵母的减数分裂

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Please note that all translations are automatically generated. Click here for the English version.

使用延时显微镜和蛋白质的阶段特异性核耗竭来研究酿酒酵母的减数分裂

DOI:

10.3791/64580-v

•

07:48 min

•

October 11, 2022

•

Gisela Cairo*¹, Anne MacKenzie*¹, Dai Tsuchiya², Soni Lacefield

¹Department of Biology,Indiana University, ²Stowers Institute for Biomedical Research

Chapters

00:05Introduction
00:42Time-Lapse Fluorescence Microscopy
05:32Analysis of Chromatin Segregation
06:18Results: Ctf19 Anchor Away Time and Chromatin Segregation
07:10Conclusion

Summary

Automatic Translation

延时显微镜是研究出芽酵母减数分裂的宝贵工具。该协议描述了一种结合细胞周期同步，延时显微镜和目标蛋白的条件耗竭的方法，以证明如何在减数分裂染色体分离期间研究特定蛋白质的功能。

Tags

Keywords: Time-lapse Microscopy Stage-specific Nuclear Depletion Meiosis Saccharomyces Cerevisiae Prophase I Conditional Protein Depletion Budding Yeast Mitosis Cell Cycle Events Nuclear Envelope Breakdown Agar Pad Monolayer Of Cells Sporulation Culture Centrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

化验粘附和酿酒酵母琼脂入侵

拆装时间推移显微镜监测肌动蛋白

双光子现场斑马鱼的胚胎干切断和时间推移共焦成像

一种高通量的方法，在全球范围内研究在酿酒酵母中的细胞器形态

鉴定与定量蛋白质组学在酿酒酵母中的蛋白质复合物

有丝分裂的siRNA转染后的时间推移成像

时间推移显微镜早期胚胎发育线虫

随机使用时移视频显微镜细胞迁移的定量分析

流式细胞仪分离纯化<em> S.酵母</em>合子

使用时间的推移显微镜可视化缺氧诱导假死<em> C.线虫</em>胚胎

Read Article