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Anvendelse af timelapsemikroskopi og fasespecifik nuklear udtømning af proteiner til undersøgelse af meiose i S. cerevisiae
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JoVE Journal Biology
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Anvendelse af timelapsemikroskopi og fasespecifik nuklear udtømning af proteiner til undersøgelse af meiose i S. cerevisiae

DOI:
10.3791/64580-v

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07:48 min

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October 11, 2022

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, , ,
1Department of Biology,Indiana University, 2Stowers Institute for Biomedical Research

Chapters

  • 00:05Introduction
  • 00:42Time-Lapse Fluorescence Microscopy
  • 05:32Analysis of Chromatin Segregation
  • 06:18Results: Ctf19 Anchor Away Time and Chromatin Segregation
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Time-lapse mikroskopi er et værdifuldt værktøj til at studere meiose i spirende gær. Denne protokol beskriver en metode, der kombinerer cellecyklussynkronisering, time-lapse-mikroskopi og betinget udtømning af et målprotein for at demonstrere, hvordan man studerer funktionen af et specifikt protein under meiotisk kromosomadskillelse.

Tags

Keywords: Time-lapse MicroscopyStage-specific Nuclear DepletionMeiosisSaccharomyces CerevisiaeProphase IConditional Protein DepletionBudding Yeast MitosisCell Cycle EventsNuclear Envelope BreakdownAgar PadMonolayer Of CellsSporulation CultureCentrifugation

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