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使用腺病毒介导的转导在天然膀胱尿氦中表达转基因
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Biology
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JoVE Journal
Biology
Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
Please note that all translations are automatically generated.
Click here for the English version.
使用腺病毒介导的转导在天然膀胱尿氦中表达转基因
DOI:
10.3791/64584-v
•
06:01 min
•
October 06, 2022
•
Wily G. Ruiz
,
Dennis R. Clayton
,
Marianela G. Dalghi
,
Nicolas Montalbetti
,
Marcelo D. Carattino
,
Gerard Apodaca
1
Renal-Electrolyte Division, Department of Medicine
,
University of Pittsburgh School of Medicine
Chapters
00:04
Introduction
00:48
Transduction of Rodent Bladder
04:08
Results: Transducing the Native Rodent Urothelium for Expression of Transgenes or Downregulation of Endogenous Gene Products
05:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述了用于生成大量重组腺病毒的方法,然后可用于转导天然啮齿动物尿路上皮,从而表达转基因或下调内源性基因产物。
Tags
Adenovirus-mediated Transduction
Bladder Urothelium
CDNA
SiRNA Expression
Catheterization
N-dodecyl-beta-D-maltoside
PBS Wash
Anesthetized Mouse
Gene Delivery
Urothelial Gene Expression
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