Journal
/
Biology
/
Ekspresjon av transgener i naturlig blære urotel ved bruk av adenovirusmediert transduksjon
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Ekspresjon av transgener i naturlig blære urotel ved bruk av adenovirusmediert transduksjon

DOI:
10.3791/64584-v

•

06:01 min

•

October 06, 2022

•

, , , , ,
1Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh School of Medicine

Chapters

  • 00:04Introduction
  • 00:48Transduction of Rodent Bladder
  • 04:08Results: Transducing the Native Rodent Urothelium for Expression of Transgenes or Downregulation of Endogenous Gene Products
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

Metoder er beskrevet for generering av store mengder rekombinante adenovirus, som deretter kan brukes til å transducere det opprinnelige gnagerurotelet som muliggjør ekspresjon av transgener eller nedregulering av endogene genprodukter.

Tags

Adenovirus-mediated TransductionBladder UrotheliumCDNASiRNA ExpressionCatheterizationN-dodecyl-beta-D-maltosidePBS WashAnesthetized MouseGene DeliveryUrothelial Gene Expression

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

Detection of Protein Ubiquitination

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

In vitro Reconstitution of the Active T. castaneum Telomerase

Genetisk kodet Molekylær Sonder til Study G protein-koblede reseptorer

Proteomikk Prøvepreparering fra formalinfiksert og parafin Embedded Tissue

Read Article