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Developmental Biology
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从人类扩增的潜在干细胞中产生自然杀伤细胞
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从人类扩增的潜在干细胞中产生自然杀伤细胞
DOI:
10.3791/64608-v
•
06:53 min
•
January 13, 2023
•
Poon Yu Ching
,
Clement Wang
,
Su Hang
,
Pentao Liu
3
,
Ryohichi Sugimura
3
1
Major in Molecular Biology and Biotechnology, School of Biological Sciences
,
The University of Hong Kong
,
2
School of Biomedical Sciences
,
The University of Hong Kong
,
3
Centre for Translational Stem Cell Biology
Chapters
00:04
Introduction
00:33
Generation of 3D Organoid Structures From hEPSCs
02:02
Mesoderm and Hemato-Endothelial Patterning of the Embryoid Body
03:07
NK Cell Differentiation
05:05
Results: Analysis of In Vitro Differentiation (IVD) Products
06:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议显示了如何在3D和2D培养条件下区分具有轻度细胞毒性的CD3 - / CD45 + CD56 +细胞与人扩增潜在干细胞(hEPSC)。这允许在不破坏复杂微环境的情况下进行常规表型验证。
Tags
Keywords: Natural Killer Cells
Human Expanded Potential Stem Cells
Cancer Immunotherapy
Embryoid Bodies
Mesoderm Patterning
Hematoendothelial Specification
Differentiation
Cell Culture
Cell Suspension
Cell Counting
Cell Harvesting
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