Journal
/
/
Generering af naturlige dræberceller fra menneskelige stamceller med udvidet potentiale
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
DOI:

06:53 min

January 13, 2023

, , , ,

Chapters

  • 00:04Introduction
  • 00:33Generation of 3D Organoid Structures From hEPSCs
  • 02:02Mesoderm and Hemato-Endothelial Patterning of the Embryoid Body
  • 03:07NK Cell Differentiation
  • 05:05Results: Analysis of In Vitro Differentiation (IVD) Products
  • 06:29Conclusion

Summary

Automatic Translation

Denne protokol viser, hvordan man kan differentiere CD3−/CD45+CD56+ celler med mild cytotoksicitet fra humane ekspanderede potentielle stamceller (hEPSC'er) under både 3D- og 2D-dyrkningsbetingelser. Dette giver mulighed for rutinemæssig fænotypisk validering uden ødelæggelse af det komplekse mikromiljø.

Related Videos

Read Article