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来自切除人肿瘤标本的 离体 器官型腹膜假粘液瘤肿瘤切片的培养和成像
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Culture and Imaging of Ex Vivo Organotypic Pseudomyxoma Peritonei Tumor Slices from Resected Human Tumor Specimens
DOI:

09:19 min

December 09, 2022

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Chapters

  • 00:04Introduction
  • 00:48Preparation of Human Pseudomyxoma Peritonei (PMP) Tissues for Tissue Processing and Culture
  • 03:07Confocal Imaging of Living Human Tissue Slices
  • 03:51Disassociation of Living Slices for Flow Cytometry
  • 05:23Pharmacological Intervention Using Living Tissue Slices for Viability and Proliferation Analysi
  • 06:31Results: Real-Time Visualization and Interrogation of Tumor Using Patient-Derived Ex Vivo Organotypic Slices
  • 08:43Conclusion

Summary

Automatic Translation

我们描述了一种用于人类癌症的生产,培养和可视化的方案,这些癌症已经转移到腹膜表面。使用振动切片机切割切除肿瘤标本,并在可渗透的插入物上培养以增加氧合和活力,然后使用共聚焦显微镜和流式细胞术进行成像和下游分析。

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