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使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像
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Neuroscience
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JoVE Journal Neuroscience
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
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使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

DOI:
10.3791/64701-v

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08:25 min

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April 21, 2023

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, , , ,
1Department of Neurology,University of Massachusetts Chan Medical School, 2Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology,University of Massachusetts Chan Medical School

Chapters

  • 00:04Introduction
  • 00:48Traumatic Brain Injury (TBI) Surgery
  • 01:58Cranial Window Implantation Surgery
  • 06:43Results: Intravital Two‐Photon Imaging
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

这项研究表明，向小鼠提供重复性创伤性脑损伤，并同时植入颅窗，以便随后使用双光子显微镜对神经元表达的 EGFP 进行活体成像。

Tags

Intravital ImagingFluorescent ProteinTraumatic Brain InjuryCranial WindowTwo-photon MicroscopyNeuronal ResponseMechanical StressGene Of InterestVirus PromoterCell TypesOphthalmic OintmentMidline IncisionPeriosteumStereotaxic FrameTBI Impact SiteImpactor TipDental CementLateral MusclesCranial Window Implantation

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