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In vivo Entrega de genes en células epiteliales mamarias de ratón a través de inyección intraductal mamaria
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection
DOI:

05:36 min

February 10, 2023

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Chapters

  • 00:04Introduction
  • 00:39Syringe and Virus Preparation
  • 01:47Animal Preparation
  • 02:27Intraductal Injection of Virus
  • 03:14Detection of Infected Cells by a Fluorescent Stereomicroscope
  • 03:58Results: Analyzing the Mammary Epithelial Cells After the Intraductal Injection
  • 04:57Conclusion

Summary

Automatic Translation

El presente protocolo describe la inyección intraductal de vectores virales a través de la tetina para administrar genes de interés en las células epiteliales mamarias.

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