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基于CRISPR的一步法黑腹果蝇内源性基因标记策略
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JoVE Journal Neuroscience
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
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基于CRISPR的一步法黑腹果蝇内源性基因标记策略

DOI:
10.3791/64729-v

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07:23 min

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January 26, 2024

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, ,
1Department of Cell and Developmental Biology,University of Michigan, 2Cellular and Molecular Biology Program,University of Michigan, 3Michigan Neuroscience Institute Affiliate,University of Michigan

Chapters

  • 00:00Introduction
  • 00:30Design and Construction of Gene Editing Reagents
  • 06:51Results: Analyzing PERIOD-mNeonGreen Protein Expression in Clock Neurons
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种简化的果蝇内源性基因标记方案，该方案利用基于PCR的技术对成功的基因修饰进行无标记鉴定，从而促进了稳定的敲入系的开发。

Tags

Keywords: CRISPRGene TaggingDrosophila MelanogasterHomologous RecombinationFluorescent ProteinKnock-inSgRNADonor VectorCloningPlasmidSanger Sequencing

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