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小鼠脉络丛的显微切割和全安装扫描电子显微镜可视化

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus

Please note that all translations are automatically generated. Click here for the English version.

小鼠脉络丛的显微切割和全安装扫描电子显微镜可视化

DOI:

10.3791/64733-v

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06:45 min

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December 16, 2022

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Elien Van Wonterghem*^1,2, Lien Van Hoecke*^1,2, Griet Van Imschoot², Daan Verhaege², Marlies Burgelman², Roosmarijn E. Vandenbroucke²

¹VIB Center for Inflammation Research,VIB, ²Department of Biomedical Molecular Biology,Ghent University

Chapters

00:05Introduction
00:59Microdissection of the Choroid Plexus Out of the Lateral and Fourth Ventricle
03:47Morphological Analysis of Choroid Plexus Tissue Using Scanning Electron Microscopy (SEM)
04:48Results: Isolation of Choroid Plexus and Analysis of Cellular Structure
05:49Conclusion

Summary

Automatic Translation

脉络丛（CP）是神经科学中研究不足的组织，在中枢神经系统的健康和疾病中起着关键作用。该协议描述了用于分离CP的显微切割技术，并使用扫描电子显微镜来获得其细胞结构的整体视图。

Tags

Keywords: Microdissection Whole Mount Scanning Electron Microscopy Choroid Plexus Brain Ventricles Staining Transcardial Perfusion Mouse Brain Cerebellum Fourth Ventricle

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