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Chapters
- 00:04introduction
- 00:45Bead Preparation and Sterilization
- 01:133D Insert Preparation Under a Sterile Hood
- 02:04Cell Line Harvesting and 3D Maintenance
- 03:32Addition of Cells of Interest into the System
- 04:073D Bone Marrow-Like Experimental Results
- 07:50Results: In Vitro 3D Human Bone Marrow-Like Microenvironment for Understanding the Resident Cell Interactions
- 08:33Conclusion
Hier beschreiben wir ein einfach zu implementierendes, standardisiertes, mikrophysiologisches System, das die Komplexität der in vivo-Struktur des menschlichen Knochenmarks widerspiegelt und ein relevantes Modell zur feinen Untersuchung eines breiten Spektrums normaler und pathologischer Ereignisse bietet.
Tags
Keywords:
3D ModelBone MarrowResident CellsPhysiological ContextTumoral ContextStandardized SystemHuman Cell LinesPrimary Cell PopulationsBone Marrow-like StructureCell HarvestIn Situ Cell Localization3D Structure DissociationCellular ComponentMolecular StudiesFunctional StudiesBCP BeadsPolycarbonate MembraneCell Culture InsertsHMEC-1 CellsHS27A CellsVascular CompartmentMesenchymal Stromal Cell CompartmentOsteoblast DifferentiationCell Suspension
