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采用多转染的细胞状态识别电路的快速开发
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Bioengineering
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JoVE Journal Bioengineering
Rapid Development of Cell State Identification Circuits with Poly-Transfection
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采用多转染的细胞状态识别电路的快速开发

DOI:
10.3791/64793-v

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09:21 min

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February 24, 2023

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, , ,
1Department of Biological Engineering,Massachusetts Institute of Technology, 2School of Biological Engineering,University of British Columbia, 3Department of Electrical Engineering and Computer Science,Massachusetts Institute of Technology

Chapters

  • 00:04Introduction
  • 00:43Transfection and Flow Cytometry
  • 04:59Results: Poly-Transfection: A High-Throughput Extension of Traditional Mammalian Transfection
  • 08:31Conclusion

Summary

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复杂的基因回路在设计、测试和优化方面非常耗时。为了促进这一过程，哺乳动物细胞的转染方式允许在单个孔中测试电路组件的多个化学计量。该方案概述了实验计划、转染和数据分析的步骤。

Tags

Cell State IdentificationPoly-transfectionSynthetic BiologyFlow CytometryReporter GenesTransfectionTransfection ReagentPlasmidFiller PlasmidReduced Serum MediumEnhancer ReagentMKO2TagBFPNeon GreenL7Ae

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