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Untersuchung der protektiven Wirkung von Platycodin D auf die nicht-alkoholische Fettlebererkrankung in einem Palmitinsäure-induzierten In-vitro-Modell
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Medicine
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JoVE Journal Medicine
Investigating the Protective Effects of Platycodin D on Non-Alcoholic Fatty Liver Disease in a Palmitic Acid-Induced In Vitro Model
DOI:

08:20 min

December 02, 2022

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Chapters

  • 00:05Introduction
  • 00:58Pretreatment of AML-12 Cells for In Vitro NAFLD Model
  • 02:32Measuring the Change in ROS Production and Mitochondrial Membrane Potential
  • 04:05Measuring the Protein Expression Levels of LC3-II/LC3-I and p62/SQSTM1
  • 05:59Results: Effects of Platycodin D on Intracellular ROS, MMP Depolarization, and Protein Expression Levels in AML-12 Cells
  • 07:49Conclusion

Summary

Automatic Translation

Dieses Protokoll untersucht die protektive Wirkung von Platycodin D auf die nicht-alkoholische Fettlebererkrankung in einem Palmitinsäure-induzierten In-vitro-Modell .

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