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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DOI:
10.3791/64833-v

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04:17 min

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May 10, 2024

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, , , , ,
1Center of Precision Medicine and Healthcare,Tsinghua-Berkeley Shenzhen Institute, 2Institute of Biopharmaceutics and Health Engineering,Tsinghua Shenzhen International Graduate School

Chapters

  • 00:00Introduction
  • 00:40Combining Recombinase Polymerase Amplification (RPA) and CRISPR/Cas12a Systems for the Detection of DNA Viruses
  • 01:20CRISPR/Cas12a Detection With/Without Smartphone Microscopy (SPM)
  • 02:23Data Augmentation and Transfer Learning
  • 02:58Results: AI-Detection of DNA Virus Using RPA-CRISPR/Cas12a-SPM
  • 03:26Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.

Tags

Keywords: DNA Virus DetectionRPA-CRISPR/Cas12a-SPMDeep LearningFrog Virus 3Point-of-care DetectionRapid And Sensitive DetectionRPA EnzymesCRISPR/Cas12a SystemSmartphone-based MicroscopeAI ClassificationAmplification EfficiencyCollateral CleavageDetection Sensitivity

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