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Cancer Research
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腹膜内移植在小鼠中产生急性髓系白血病
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Intra-Peritoneal Transplantation for Generating Acute Myeloid Leukemia in Mice
Please note that all translations are automatically generated.
Click here for the English version.
腹膜内移植在小鼠中产生急性髓系白血病
DOI:
10.3791/64834-v
•
10:02 min
•
January 06, 2023
•
Fenghua Qian
,
Brooke E. Arner
,
Shaneice K. Nettleford
,
Robert F. Paulson
,
K. Sandeep Prabhu
1
Department of Veterinary and Biomedical Sciences, Center for Molecular Immunology and Infectious Disease and Center for Molecular Toxicology and Carcinogenesis, Penn State Cancer Institute
,
The Pennsylvania State University
Chapters
00:05
Introduction
00:50
Lentiviral Transduction
04:18
Serial Transplantation
07:22
Results: Comparing Transplantation Efficiency of Murine AML Cells Using Retro-Orbital and Intra-Peritoneal Routes of Transplantation
09:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,腹膜内注射白血病细胞用于在小鼠中建立和繁殖急性髓性白血病(AML)。这种新方法在AML细胞的连续移植中是有效的,并且可以作为那些可能在小鼠静脉注射中遇到困难和不一致的人的替代方案。
Tags
Intra-peritoneal Transplantation
Acute Myeloid Leukemia
MLL-AF9
Mouse Model
Bone Marrow
Cell Isolation
CD45.1
C57BL/6J
Surgical Procedure
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