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Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
 

Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Article DOI: 10.3791/64846-v • 05:28 min • June 2nd, 2023
June 2nd, 2023 •
1, 1, 2, 3, 4, 1
1Swiss Federal Institute of Technology Lausanne (EPFL), 2Division of Genetics, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, 3Department of Pharmacology and Toxicology, College of Pharmacy, University of Utah, 4University of Lausanne (UNIL)

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Summary
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Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages. Here, we couple standard assays in zebrafish with Z-REX for signaling pathway analysis.

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Keywords: Zebrafish Z-REX Signaling Responses Electrophilic Drugs Metabolites Reactive Ligand Target Engagement Phenotype Neutrophils Macrophages Precision Isoform-specific Kinase Inhibitors Triple-negative Breast Cancer
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