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Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

DOI:
10.3791/64874-v

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12:54 min

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May 19, 2023

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, , , , , , ,
1Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture,International Atomic Energy Agency, 2Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health,University of Veterinary Medicine, Vienna

Chapters

  • 00:04Introduction
  • 01:09Production of Naive Bovine Monocyte-Derived Dendritic Cells (MoDCs)
  • 06:01Generation of Antigen-Pulsed MoDCs
  • 07:20MoDC Lymphocyte Co-Culture
  • 08:20Flow Cytometric Analysis
  • 09:47Results: In Vitro Generation of Cattle MoDCs for the Evaluation of Candidate Vaccine Antigens
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle.

Tags

Keywords: BovineMonocyte-derived Dendritic CellsVaccine ImmunogenicityIn Vitro AssayAntigen-presenting CellsVaccine EfficacyQuality ControlAdjuvant SelectionPeripheral Blood Mononuclear CellsCD14Flow Cytometry

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