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A Simple Protocol for Mapping the Plant Root System Architecture Traits
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JoVE Journal Biology
A Simple Protocol for Mapping the Plant Root System Architecture Traits

A Simple Protocol for Mapping the Plant Root System Architecture Traits

DOI:
10.3791/64876-v

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11:09 min

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February 10, 2023

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, ,
1Department of Biology,Western Kentucky University, 2Plant Biotechnology Division,CSIR Central Institute of Medicinal and Aromatic Plants, 3Academy of Scientific and Innovative Research (AcSIR), 4Department of Biology,St. Joseph’s University

Chapters

  • 00:04Introduction
  • 00:52Arabidopsis Seed Surface Sterilization
  • 02:23Setting a Hydroponic System for Seed Germination
  • 03:39Examination of Root System Architecture (RSA)
  • 04:07Recording Images for Root System Architecture (RSA)
  • 07:45Results: Mapping the Plant Root System Architecture Traits
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.

Tags

Keywords: Plant Root System ArchitectureNon-invasive Hydroponic MethodRoot System VisualizationArabidopsis SeedsSurface SterilizationPolycarbonate SheetPolypropylene MeshMS Basal MediaHydroponicsRoot Growth

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