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颅内插管植入小鼠连续局部区域嵌合抗原受体（CAR）T细胞输注

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

Please note that all translations are automatically generated. Click here for the English version.

颅内插管植入小鼠连续局部区域嵌合抗原受体（CAR）T细胞输注

DOI:

10.3791/64886-v

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05:22 min

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February 24, 2023

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Kyra Harvey, Peter J. Madsen³, Tiffany Smith, Crystal Griffin², Luke Patterson², Nicholas A. Vitanza⁵, Phillip B. Storm³, Adam C. Resnick³, Jessica B. Foster²

¹Division of Oncology,Children’s Hospital of Philadelphia, ²Center for Data Driven Discovery in Biomedicine,Children’s Hospital of Philadelphia, ³Division of Neurosurgery,Children’s Hospital of Philadelphia, ⁴Ben Towne Center for Childhood Cancer Research,Seattle Children’s Research Institute, ⁵Department of Pediatrics, Seattle Children’s Hospital,University of Washington

Chapters

00:04Introduction
00:48Mouse Preparation and Surgery Procedure
03:47CAR T Cell Infusion
04:36Results: Efficacy of CAR T Cell Therapy
04:53Conclusion

Summary

Automatic Translation

中枢神经系统（CNS）肿瘤是儿童癌症相关死亡的主要原因，基于局部免疫的疗法越来越多地在临床试验中为患者进行测试。该协议描述了在小鼠中植入局部区域套管的方法，用于靶向CNS肿瘤的免疫治疗输注的临床前评估。

Tags

Keywords: Intracranial Cannula CAR T Cell Locoregional Delivery Intraventricular Catheter Stereotactic Surgery Stereotaxic Apparatus Cranial Landmarks Acrylic Resin Repeated Dosing Neuroscience Research

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