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Journal
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Chemistry
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光控生物活性化合物的
体外
和
体内
评价 - 癌症光药理学的潜在候选药物
JoVE Journal
Chemistry
Author Produced
This content is Free Access.
JoVE Journal
Chemistry
In Vitro
and
In Vivo
Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology
Please note that all translations are automatically generated.
Click here for the English version.
光控生物活性化合物的
体外
和
体内
评价 - 癌症光药理学的潜在候选药物
DOI:
10.3791/64902-v
•
13:17 min
•
September 29, 2023
•
Kateryna Horbatok*
1,2
,
Tetyana Makhnii*
2
,
Viktoriia Kosach
,
Volodymyr Danko
,
Andrey Kovalenko
,
Stanislav Fatiushchenkov
,
Petro Borysko
,
Iryna Pishel
,
Oleg Babii
4
,
Anne S. Ulrich
,
Tim Schober
,
Sergii Afonin
,
Igor V. Komarov
2,4
1
Taras Shevchenko National University of Kyiv
,
2
Bienta/Enamine
,
3
Karlsruhe Institute of Technology
,
4
Lumobiotics
Chapters
00:00
Introduction
01:22
IC
50
Evaluation for LMB002 (“ring-closed” and “ring-open” forms) Using 2D and 3D LLC Cell Cultures
03:06
2D Cell Culture Experiment—Adding the Compounds (Day 2)
04:14
2D Cell Culture Experiment—Staining and Imaging (Days 2–5)
04:45
3D Cell Culture Experiment
05:46
Determination of Photoswitching Efficiency in a Tissue Surrogate
07:46
In Vivo Anticancer Efficacy Determination
09:39
Representative Results
12:40
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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português (Portuguese)
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Automatic Translation
该协议提供了一组用于评估可光开关抗癌肽的实验,可用于此类化合物的临床前筛选。这包括 2D 和 3D 细胞培养物中的细胞毒性评估、
离体
(模型组织)光异构化效率和
体内
功效的评估。
Tags
Photocontrolled Compounds
Photoswitchable Drugs
Photopharmacology
In Vitro
In Vivo
Cancer
Cell Viability Assay
Lewis Lung Carcinoma
DMSO
DMEM
Photoirradiation
Laser
Article
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