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Bioengineering

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JoVE Journal Bioengineering
Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
 

Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells

Article DOI: 10.3791/64909-v • 10:08 min • June 16th, 2023
June 16th, 2023 •
1, 1, 2, 1, 1, 1,2,3,4, 1,2,4
1Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, 2Department of Biomedical Engineering, Samueli School of Engineering, University of California, Irvine, 3Department of Chemistry, School of Physical Sciences, University of California, Irvine, 4Sue & Bill Gross Stem Cell Research Center, University of California, Irvine

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Summary
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Here, we present light-induced dielectrophoresis as a label-free approach for characterizing heterogeneous cell lines, specifically human mesenchymal stem cells (hMSCs). This paper describes a protocol for using and optimizing a microfluidic device with a photoconductive layer to characterize the electrical behavior of hMSCs without altering their native state.

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Keywords: Light-induced Dielectrophoresis Mesenchymal Stem Cells Heterogeneity Virtual Electrodes Microfluidic Device ITO Glass Photoconductor Copper Tape
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