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1Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Tianfu Chinese Medicine Innovation Harbour,Chengdu University of Traditional Chinese Medicine, 3School of Medical Technology,Chengdu University of Traditional Chinese Medicine, 4China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd.
Chapters
- 00:04Introduction
- 01:02Sample Preparation
- 01:55Installation of Desorption Electrospray Ionization (DESI) Unit
- 04:02Desorption Electrospray Ionization‐Mass Spectrometry (DESI‐MS) Image Acquisition
- 05:50Processing DESI‐MSI Data and Visualization
- 06:55Results: Mass Spectrometry Imaging of Root and Whole Leaf Sections
- 07:31Conclusion
En este estudio, se presentan una serie de métodos para preparar muestras DESI-MSI de plantas, y se describe en detalle un procedimiento de instalación de ensamblaje DESI, adquisición de datos MSI y procesamiento. Este protocolo se puede aplicar en varias condiciones para adquirir información de metaboloma espacial en plantas.
