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Valutazione dei livelli di autofagia in due diversi modelli di cellule pancreatiche utilizzando l'immunofluorescenza LC3
JoVE Journal
Biology
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JoVE Journal Biology
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
DOI:

08:07 min

April 28, 2023

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Chapters

  • 00:05Introduction
  • 00:49PANC-1 Cell Preparation and Gemcitabine Treatment
  • 01:56Fixation, Permeabilization, and Blocking the PANC-1 Cells
  • 02:32Antibody Incubation
  • 03:57DAPI Staining, Montage, and Visualization of the Cells
  • 04:51LC3 Dots Quantification
  • 06:13Results: Effect of LC3 Immunofluorescence in Pancreatic Cell Lines
  • 07:26Conclusion

Summary

Automatic Translation

L'obiettivo di questo protocollo è determinare i livelli autofagici nel cancro del pancreas e nelle cellule acinose pancreatiche attraverso l'immunofluorescenza LC3 e la quantificazione dei punti LC3.

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