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Adipocyt-specifieke ATAC-Seq met vetweefsels met behulp van fluorescentie-geactiveerde kernsortering
JoVE Journal
Genetics
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JoVE Journal Genetics
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
DOI:

11:11 min

March 17, 2023

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Chapters

  • 00:05Introduction
  • 00:51Nucleus Isolation
  • 02:15Nucleus Sorting and Tn5 Tagmentation
  • 04:08DNA Purification and PCR Amplification
  • 05:32Real-Time Quantitative PCR (qPCR) Test, Additional PCR Amplification, and Second DNA Purification
  • 06:14DNA Fragment Size Selection
  • 08:01Results: Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing
  • 10:19Conclusion

Summary

Automatic Translation

We presenteren een protocol voor assay voor transposase-toegankelijk chromatine met high-throughput sequencing (ATAC-seq) specifiek op adipocyten met behulp van kernsortering met vetweefsels geïsoleerd uit transgene reportermuizen met nucleaire fluorescentielabeling.

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