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使用刺胞动物模式生物Clytia hemisphaerica表征体内上皮伤口愈合
JoVE Journal
Biology
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JoVE Journal Biology
Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
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使用刺胞动物模式生物Clytia hemisphaerica表征体内上皮伤口愈合

DOI:
10.3791/65081-v

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07:47 min

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February 10, 2023

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, ,
1Department of Molecular Genetics and Cell Biology,The University of Chicago

Chapters

  • 00:05Introduction
  • 00:53Procedure for Wounding: Microwounds and Small Wounds
  • 02:13Procedure for Wounding: Large Wounds
  • 03:24Imaging Epithelial Wound Healing
  • 03:52Procedure for Mesogleal Injections
  • 05:32Results: The Epithelial Wound Healing Process
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了一种在活 的Clytia hemisphaerica medusa 的上皮中创建伤口的方法，并在 体内以高分辨率图像伤口愈合。此外，还提出了一种在伤口愈合过程中引入染料和药物来扰乱上皮细胞和细胞外基质中的信号传导过程的技术。

Tags

Epithelial Wound HealingClytia HemisphaericaIn VivoLive ImagingMicro-woundsScratch WoundsMicroinjectionCell MigrationCell SpreadingExtracellular MatrixCell Cytoplasm

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