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使用基于光学的系统对水凝胶嵌入的完整小鼠肌纤维进行高通量收缩测量
JoVE Journal
Biology
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JoVE Journal
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High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System
Please note that all translations are automatically generated.
Click here for the English version.
使用基于光学的系统对水凝胶嵌入的完整小鼠肌纤维进行高通量收缩测量
DOI:
10.3791/65103-v
•
07:35 min
•
May 05, 2023
•
Leander A. Vonk*
1
,
Osman Esen*
1
,
Michaela Yuen
4
,
Tyler J. Kirby
2,3
1
Department of Physiology
,
Amsterdam UMC
,
2
Amsterdam Cardiovascular Sciences, Heart Failures and Arrhythmias
,
Amsterdam UMC
,
3
Amsterdam Movement Sciences, Tissue Function and Regeneration
,
Amsterdam UMC
,
4
Discipline of Child and Adolescent Health, Faculty of Health and Medicine
,
University of Sydney
Chapters
00:04
Introduction
00:58
Murine Flexor Digitorum Brevis Muscle Dissection
02:07
Muscle Digestion, Trituration, and Gravity Sedimentation
02:59
Fiber Embedding
03:44
Optics‐Based Contractile Measurements
06:20
Results: Embedded Muscle Contraction Assessment
06:59
Conclusion
Summary
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Automatic Translation
骨骼肌功能可以通过量化孤立的肌肉纤维的收缩力来评估,传统上使用费力的低通量方法。在这里,我们描述了一种基于光学的高通量方法来量化水凝胶嵌入的肌肉纤维的收缩性。这种方法在药物筛选和治疗开发方面有应用。
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