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使用体内显微注射和电穿孔的体细胞基因组工程小鼠模型

JoVE Journal
Cancer Research

This content is Free Access.

JoVE Journal Cancer Research

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Please note that all translations are automatically generated. Click here for the English version.

使用体内显微注射和电穿孔的体细胞基因组工程小鼠模型

DOI:

10.3791/65131-v

•

08:06 min

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May 05, 2023

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Keerthana Harwalkar², Nobuko Yamanaka³, Yojiro Yamanaka^2,3

¹Rosalind and Morris Goodman Cancer Research Institute,McGill University, ²Department of Human Genetics,McGill University, ³McGill Integrated Core for Animal Modeling (MICAM),McGill University

Chapters

00:05Introduction
01:01Preparing Microinjection Needle, Surgery Area, Solution, and Needle for Injection
02:26Exposing the Female Reproductive Tract
04:11In Vivo Oviduct Injection and Electroporation
06:29Results: Validation of Successful In Vivo Injection and Electroporation of Oviduct Epithelial Cells
07:16Conclusion

Summary

Automatic Translation

该协议描述了显微注射和体内电穿孔，用于小鼠输卵管中区域限制的CRISPR介导的基因组编辑。

Tags

Keywords: Somatic Genome Engineering Mouse Models In Vivo Microinjection Electroporation Organ Targeting Cancer Initiation Genome Manipulation Immunocompetent Environment Adaptable Mutation Combinations Oviduct Targeting Organ Parenchyma Targeting Glass Capillary Microinjection Anesthesia Aseptic Technique

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